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Subsequent to the TLR-ligand interaction, APCs (mainly DCs) uptake and process vaccine antigens to be exposed on cell membrane surface in association to major histocompatibility complex (MHC) molecules for efficient presentation to adaptive immune cells [ 24].
Our atomic model, combined with the aforementioned genetic and biochemical studies, suggests that the reduction, abolition and frame shifting of the trans-translation activity is due to the incorrect presentation of the resume codon to the A site, subsequent to the alteration of the interaction between the SmpB and the resume codon upstream region.
Nongenomic effects are probably subsequent to cytosolic interaction between the glucocorticoid receptor and proteins, or to interaction between glucocorticoids and specific membrane binding sites.
Quiescent T lymphocytes reorganize the actin cytoskeleton subsequent to interaction with antigen presenting cells bearing the appropriate peptide antigen.
Subsequent to docking, the best interaction pose was selected using our scoring scheme DockScore, which ranks the docked solutions based on several interface parameters and aims to find optimal interactions between proteins.
Whereas the ectodomain of US2 is responsible for the initial recognition of target MHC I molecules, its TMS and cytosolic tail are necessary to complete subsequent steps of the interaction, such as forwarding MHC I for proteasomal degradation.
Our aims are (1) to assess the influence of the effect of life events on first and recurrent admissions; (2) to assess the influence of prior admissions on the risk of subsequent admissions; and (3) to test the interaction between life event load and number of admissions (i.e. as indication for a kindling effect) in those twins with bipolar disorder.
Due to poor expression and stability of the Tob W93A mutant, we were unable to test the interaction of this mutant with CNOT7 in vitro, although the mutant expressed in mammalian cells for subsequent interaction assays in vivo.
The kinetics of surface pressure evolution due to subsequent peptide interaction with the monolayer was recorded.
Based on this result, we used FZD7R in all subsequent assays to further analyze the interaction between Daple and FZDR.
These results imply that CCIs are essential for the initial complex formation between AsGM1 and the integrin and that subsequent CPI (carbohydrate-to-protein interaction) completes the complex formation.
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