Homology models were constructed with the heteromeric nACh receptor structure from T. marmorata as template (PDB 2bg9; Unwin, 2005) using Modeller v8.2 (Sali and Blundell, 1993) subsequent to sequence alignment with CLUSTAL W (Thompson et al., 1994).
U00096.2) [ 51] – including updates made subsequent to sequence publication through the ASAP [ 43] and Ecocyc [ 41] databases – and orthologous protein annotations in V. cholerae N16961 (GenBank accession nos. AE003852.1, AE003853.1) [ 34].
Subsequent to genome sequencing, a large number of microarray, proteomic and ChIP-chip assays were carried out to reconstruct the genetic network.
Subsequent to our sequence analysis, we found that 3022 scaffolds did not match with soybean sequences.
Subsequent to multiple sequence alignment, it became evident that several proteins appeared to be catalytically inactive, owing to loss or mutation(s) in residues believed to be essential for catalysis.
Subsequent matches to sequence databases showed these features to be correct.
PCR for part of the 16S rRNA gene resulted in a 528 basepair fragment which by DNA sequencing and subsequent comparison to sequences deposited in the NCBI database showed sequence homology with Treponema pallidum (505 out of 507 identical bases).
It is activated by phosphorylation at two types of residues, that is, Tyr and Ser, allowing homo- and/or heterodimerization, translocation into the nucleus and subsequent binding to sequence-specific DNA motifs.
Subsequent to confirmation by DNA sequencing, the mutated sequences were recombined into the various expression constructs as described above.
All patients were scanned with a specialized T1 MRI sequence subsequent to their clinical scan.
The same has been confirmed in the tobacco (genus Nicotiana) including the losses of some retrotransposon sequences subsequent to the allotetraploid tobacco's formation [81], [82].
