Removal of heavy debris in the same size range as the cysts (when performed subsequent to size separation) is carried out through density separation in brine.
PacBio sequencing of the closely related species P. reichenowi identified the presence of both insert blocks, indicating the deletion event occurred subsequent to the separation of these two species.
These systems were then successfully applied to the analysis of mixtures of cations (Li+, Na+, K+, NH4+, Ca2+, Mg2+) and anions (F−, Cl−, NO2−, NO3−, SO42−, PO43−) subsequent to chromatographic separation.
The emergence of the SPANX-A/D gene subfamily is a more recent event, subsequent to the separation of the hominoid lineage from orangutan and rhesus macaque.
It is within Cercopithecini, subsequent to the separation of Miopithecus and Allenopithecus, that our results seem to be entirely novel and unexpected.
Strictly, we cannot exclude an independent origin of subterranean habits in the Sardinian Ovobathysciola group, or drifting of a Bathysciola-like endogean ancestor to the island subsequent to its separation from the continent, but these seem to be unlikely scenarios.
For arenosa our IM analyses suggest that the diploids and the tetraploids are two distinct groups, with some migration from the diploid to the tetraploid level subsequent to the separation of the two gene pools.
Such deviations in the age distributions of elements of shared ancestry are not an artifact of our methods of LTR identification but can be expected if the loss rates of elements from two lineages deviate subsequent to their separation.
The absence of any orthologous sequences of the cytosolic FBP from PCDs, which are instead located in the eukaryotic part of the tree, might result from incomplete sequence sampling or be due to an independent replacement subsequent to the separation of Perkinsea.
In all, 505 animals derived from 10 taurine and 3 zebu cattle breeds were genotyped across more than 770,000 SNP markers to investigate the genomic changes subsequent to the separation between taurine and zebu cattle, which occurred at a date between 330 thousand and 2 million years ago [ 1, 2].
