These data suggest the existence of a cooperative interaction between the hepacivirus 5' and 3' NTRs and NS5A that is essential for a step in the virus life cycle subsequent to genome replication, most likely particle assembly.
The data suggest that in vivo fitness of the domain III chimeric virus is dependent on a cooperative interaction between the 5'NTR, 3'NTR and NS5A at a step in the viral life cycle subsequent to genome replication, most likely during particle assembly.
These results showed that the delayed life cycle of attB-Ads must be due to alterations in subsequent steps to replication.
It has been recently proposed that a precursor form of VPg (either 3AB or 3BC) could act as the authentic protein primer molecule, while processing and release of 3B (VPg) would be a step subsequent to initiation of replication, although these hypotheses are still under discussion [16], [17], [19].
Selection of SNP associations, robust to conservative Bonferroni correction, to be taken forward to subsequent replication stages, often leads to a bias towards selecting SNPs whose effect sizes in the discovery cohort are over-estimated, albeit truly positive (20).
Statistical tests comparing correlations between the multimorbidity measures and the outcomes was not reported for the subsequent analyses due to replication of results.
Since DENV replicates in vivo but UV-DENV does not, the number of virus particles is expected to be higher in mice administered DENV than UV-DENV, subsequent to the first round of replication.
The molecular mechanism for ATM activation following aphidicolin-induced replicative stress, and the subsequent localization of 53BP1 to replication sensitive sites is not known.
Although the experiment depicted was carried out in the absence of ribavirin, identical results were obtained in the presence of the drug indicating that the stimulatory capacity of ssRNA fractions is not due to subsequent replication and formation of dsRNA (data not shown).
CDK phosphorylation of yeast Sld2 and Sld3, and their subsequent binding to another replication protein Dpb11, lead to replication initiation (Tanaka et al. 2007; Zegerman & Diffley 2007; Tanaka & Araki 2010).
Deamination of C-to-U or 5meC-to-T and subsequent DNA replication or misrepair results in a C-to-T transition mutation biased to CpG dinucleotide motifs.
