Our finding of a significantly decreased risk of brain and spinal tumours in relation to ward level population mixing based on incomers from outside the region, especially among older children, may be a chance finding subsequent to multiple hypothesis testing.
To remove invariant transcripts that could contribute to multiple testing errors in the subsequent statistical analysis, downstream data filtering of the array dataset (15,208 probes) was performed.
P-values were adjusted to multiple testing by Bonferroni correction.
Significances for single factor combinations were identified by Wilcoxon pairwise tests with subsequent Holm correction for multiple tests [ 98].
We conducted t-tests with subsequent Benjamini Hochberg multiple testing correction on the MA and SCO phenotypes of this subset between normal/AZFc samples.
Unlike gene expression data for which both the technologies and methods have matured, GWAS data analysis presents numerous challenges, including testing millions of SNPs per sample and subsequent multiple test corrections, complex local linkage disequilibrium (LD) structures, and heavy computational duties due to thousands of samples, especially in permutation analysis.
This consisted of Edward's background correction [ 40], global loess normalization [ 41], and statistical analysis using linear models and empirical Bayes methods with subsequent multiple testing correction using Benjamini-Hochberg's method [ 20] (adjusted p < 0.05 was considered significant).
The subsequent univariate analysis with p-values corrected for multiple tests (Bonferroni procedure) appointed to the significant difference between normotensive and hypertensive individuals for Factor 2 (p = 0.018), which emphasized the role of ether lipids in the pathogenesis of hypertension.
For each expert-curated pathway in the database, potential differential expression was determined by a binomial test, with subsequent Bonferroni correction for multiple testing, using the PANTHER human gene reference list matching our microarrays and lists of differentially expressed genes that passed an multiple-testing corrected threshold of FDR <0.05.
Parametric data were analyzed by ANOVA with subsequent Games-Howell post-hoc test to correct for multiple testing or using the Welch test.
Parametric data were analyzed by ANOVA with subsequent Games-Howell post-hoc test to correct for multiple testing or by using the Welch test.
