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All bryostatin that has been used clinically has been sourced from one GMP isolation in 1991, and all subsequent efforts to isolate more (through isolation from source organism, aquaculture, engineered biosynthesis, or total synthesis) have been unsuccessful or not scalable.
Subsequent efforts to isolate bryostatin and replenish this supply have proven either unsuccessful or not scalable.
We do not include subsequent periods in order to isolate the estimation of this labor impact of the AUH from the potential labor incidence of the program PROGRESAR implemented in 2014.
Much effort has been put into the development and subsequent refinement of methods to isolate biologically meaningful information from the background noise [ 27- 30].
Then trapped integrants were subjected to subsequent rounds of cell sorting to isolate cells harboring gene traps with repressible promoters and reporter genes.
The remaining homogenate was used to isolate mitochondria by subsequent centrifugations [8], [22].
No attempt was made to isolate homozygous lines from subsequent Basta-selected progeny.
U.S. strains isolated subsequent to 1949 were considered to be post-vaccination era strains.
The present study was designed to isolate and characterize zinc solubilizing bacteria (ZSB) from the rhizosphere of rice and subsequent evaluation of these isolates for plant growth promotion of rice seedlings.
Of the 5 PHAEDRA isolates classified as moderately fit, 3 isolates were obtained from plasma collected 52 weeks subsequent to Baseline, and 2 isolates were obtained from plasma collected at Baseline (Figure 6).
Subsequent to tissue harvest, MSCs were isolated either by enzymatic digestion with collagenase or by explant technique.
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