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Subsequent Nco I digestion was used to insert NS5A1-31 immediately upstream, in-frame to rat MVP cDNA previously cloned into pFastBac-1 vector using standard molecular biology techniques.

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(The terms Paleocene and Oligocene were coined subsequent to Lyell's work and inserted in the lower part of the Cenozoic stratigraphic scheme).

(The terms Paleocene and Oligocene were coined subsequent to Lyell's work and inserted in the lower part of the Cenozoic stratigraphic scheme). The Neogene spans the Miocene and Pliocene epochs, and the Quaternary includes the Pleistocene and Holocene epochs.

Heterogeneous minicircle sequences were distributed randomly among families, with diversity increasing due to subsequent rearrangement of inserted fragments.