Sentence examples for subsequent to fixation from inspiring English sources

Microtissues were harvested from 384 well-plates and washed twice in 1x PBS (Sigma P7059) subsequent to fixation in 3.7% paraformaldehyde (PFA) (Sigma F1635) at 4 °C for one hour.

Subsequent to fixation in 10% buffered formalin and standard processing to paraffin wax blocks, sections (5  μm) were prepared and stained with H&E.

Evidence for this has recently been gathered from an assessment of pERK and pAKT in core-cut biopsies and pieces of surgical excisions taken at the time of operation or subsequent to routine fixation [ 4].

To distinguish the real fixation or death of a polymorphism from artifacts of the finite sampling process, sample allele frequencies subsequent to the apparent fixation or death were examined.

In this case, genetic drift is unlikely to have brought alleles to fixation before subsequent divergence [ 1, 6].

The concept of recurrent selective sweeps (often termed arms race dynamics) consists of two steps: the de novo appearance of a beneficial allele (e.g., by mutation or immigration) and its subsequent spread through the population to fixation (i.e., the selective sweep).

The influence of ethanol fixation on the stainability was therefore compared to PFA fixation with subsequent ethanol fixation to investigate which fixation procedure yields better results (data not shown).

Therefore, "producing" a work refers to the initial materialization and "reproducing" it refers to any subsequent material fixation that is modelled (in the causal sense) on its first fixation.

Soft tissue reconstruction and subsequent definitive fixation led to union of all fractures with good function.

Then, lots of 30 larvae were collected and inverted in PBS to help subsequent fixation and homogenization.

We believe that the higher rate of Zone 4 radiolucencies can be explained by the inability to place and pressurize cement on the posterior condyles of the femur without pushing it to the back of the knee during implant impaction and the subsequent difficulty to obtain fixation with an inadequate cement mantle at this location.