Subsequent to removal of duplicated reads, coverage metrics of target bases were calculated using the Picard tools.
Subsequent to the genome duplication, the number of observed duplicated transcribed genes is expected to decrease as, over time, one of the duplicates becomes transcriptionally inactive.
In total, it is estimated that at least 2.7% of the total genome has been uniquely duplicated subsequent to the human-chimpanzee split [10]; this number does not factor either deletions or small insertions into the total amount of divergence and therefore represents a minimum estimate.
OrthoMCL also identifies inparalogs (genes duplicated subsequent to speciation [ 16]) to be included in orthologous groups as within-species Blast hits that are reciprocally better than between-species hits [ 24].
These two observations taken together suggest that, on average, LSDs are relatively young compared with population duplicates, and subsequent to their generation, they display a burst of evolutionary change.
Subsequent to the emergence of a new function in one of the duplicates, selection or drift can lead to fixation of that function.
Of course, it should be noted that many of these models of gene duplicate retention and evolution can act in concert or subsequent to one another [ 94].
Partitioning of duplicate gene expression in response to environmental stress may lead to duplicate gene retention during subsequent evolution (Liu and Adam [2007]).
Although subsequent projects were of a similar high quality, the innovation of "Maxinquaye" was impossible to duplicate.
However, nucleotides artifactually introduced during the initial round of PCR amplification cannot be accurately detected as errors, even with a tagging technique, if the artifactual mutation is propagated to all subsequent PCR duplicates [ 46].
Subsequent homology analyses of oriP and EBNA1 provide more details on how this virus uses ORC and associated factors to duplicate its genome.
