Those alterations, which do not overlap, would have to occur subsequent to differentiation.
We would therefore hypothesise that loss at 17p occurred within a 'stem cell/precursor cell', while alterations at other loci occurred subsequent to differentiation into the two epithelial cell types.
Subsequent to differentiation, maturation was induced by addition of a cytokine cocktail, and after 2 days, the capacity of the matured cells to stimulate T-cell proliferation in an allogeneic (allo MLR was determined.
Subsequent to erythroid differentiation, markers of the derived-erythroid cells were analysed on day 15 using CD34-PE, CD45-APC, CD71-FITC and CD235a-PE (BD Biosciences, USA).
This suggests that LINE-1 activity and evolution occurred in the ancestral Paenungulate genome subsequent to their differentiation from other afrotherians but before the hyrax/manatee/elephant split [11], [14], [23], [24] which could not be resolved using our LINE-1 investigations.
Subsequent to the differentiation into myotubes feasibility of patch-clamp recordings of voltage gated ion channels was successfully.
Subsequent to photoreceptor differentiation, these transcription factors then become rhythmically expressed, suggesting an alternative role for these genes in photoreceptor maturation, maintenance and/or function.
In postnatal stages, Top2b expression was maintained in postmitotic cells of both neuronal and glial origin, including Rhodopsin+ rod photoreceptors (Fig. 1F), Pkcα+ bipolar cells (Fig. 1G), and EAAT1+ Müller glia (Fig. 1H), subsequent to their differentiation.
Examples include more rational management and antibiotic choice subsequent to earlier differentiation of Gram-positive cocci in clumps into MRSA, MSSA, or CNS, and earlier speciation of Gram-negative organisms.
This behaviour indicates that these genes might play a role in the establishment of the initial population of NPs, but are switched-off afterwards to allow the subsequent progression to differentiation.
Some protocols include combination of RA and testosterone [ 45] or subsequent exposure to differentiation cocktail containing forskolin, human leukemia inhibiting factor (LIF), bFGF, and CYP26 inhibitor R115866 [ 22].
