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Subsequent to data analysis, luciferase data were normalised to mean PE fluorescence signals.
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Subsequent to microarray data analysis a set of genes was chosen for validation by qPCR based on their degree of expression change, p-value, and/or known effects of the toxin studied.
The recordings were re-referenced to an average montage prior to subsequent data analysis, which was carried out in the Matlab environment from MathWorks.
All focus group discussions were facilitated by the same moderator, and an assistant moderator was also present to make notes to aid subsequent data analysis.
Stimulations were performed with a rainbow trout macrophage cell line RTS-11 [ 49], expected to be highly responsive to both cytokines, as the model used, to keep intra assay variation low and to allow maximal power to the subsequent data analysis.
Because of the high sensitivity of this analytical platform and the complex nature of metabolic samples, LC-MS experiments result in large numbers of signals that are due to unknown compounds and whose assignment forms a major challenge to the subsequent data analysis.
Team members elucidated major themes to inform subsequent data analysis.
These remaining signals after filtering are used to perform subsequent data analysis.
The data arrays built by the algorithms described above were stored in a database to facilitate subsequent data analysis, which was performed using the Weka-3 package for data mining [ 19] and direct queries to the database.
It involves the use of a novel custom-designed extensometer to measure the force displacement behaviour of skin and subsequent data analysis to estimate the shrinkage.
The output of the Master8 stimulator was sent to the CED Power 1401 and recorded in Spike2 together with the signals in order to trigger the subsequent data analysis.
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