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In addition, the users can export the module network as XGMML, GraphML and SIF file for other network tools for subsequent additional analysis.
Details and additional analysis from the earnings call to follow.
IL-1β is an additional cytokine secreted by glioma cells subsequent to TNF-α treatment.
As a result, 5,865 unigenes were referred to as differentially expressed genes (DEGs) and used for the subsequent analysis (Additional file 6).
Metabolism was then modularized by grouping core reactions with metabolically correlated functions using hierarchical clustering; this modularization enables us to look at modules of reactions as functional unities instead of individual reactions, thereby simplifying our subsequent analysis (Additional file 1).
As the 2 years of data are highly correlated and showed similar trends the 2016 data was used in the subsequent analysis (Additional file 1: Table S4).
Following filtering, 15,988 unique features were considered for subsequent analysis (Additional file 1).
One sample (diseased individual "4") was identified as an outlier and removed from subsequent analysis (Additional file 9).
Of the 1,185 amplicons, 7 did not generate sufficient numbers of reads (N < 1,000; PCR failures) and were excluded from subsequent analysis (Additional file 1).
After adaptor trimming and removal of low quality reads and orphan sequences (single reads), 19,424,182 high quality sequence reads were retained for subsequent analysis (Additional file 1).
After merging of overlapping maps, a final set of consensus maps was output and used for subsequent analysis (Additional file 2: Figure S3).
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