This in vitro model of hepatocellular lipid accumulation has been applied already in several subsequent studies to assess the effect of hepatic steatosis on the expression of defined genes and signal transduction pathways (Wanninger et al. 2011; Schnabl et al. 2011; Kirovski et al. 2010).
According to this iterative model the theory-based development of the complex intervention is followed by exploratory feasibility studies to detect, check and modify potential limitations in the study design before potentially planning and conducting a subsequent evaluation study to assess the intervention's effectiveness, if possible in a randomized controlled trial (RCT).
3. Phase III will consist of a cluster randomised trial to assess the effectiveness of the intervention, a costing study to assess its cost-effectiveness, and a subsequent qualitative study to identify barriers and facilitators in delivering the intervention.
Thus far, there has been only one analysis of a community-based study to assess the risk of comorbidity on subsequent mortality.
We performed a retrospective cohort study to assess the prevalence of such remote areas of signal change and their subsequent clinical and radiological outcome.
Applying more complex models in subsequent studies aiming to assess the number/burden of SHEs in some populations would be more difficult because it would require knowing the values of other explanatory variables to input them in the model.
Larger cattle populations will need to be assessed in subsequent studies to confirm these results and to also re-examine some of the highly ranked genes that were apparently monomorphic as the process used for gene selection was likely to exclude low frequency allelic variation.
A subsequent study will assess whether the Utstein factors explain differences in survival between emergency medical services (EMS) systems.
However, our study cannot separate drug effects from subject effects, and these exploratory results require confirmation in subsequent studies, specifically designed to assess drug effects in more detail.
Considering the marked effect of C-6 treatment on mitochondrial morphology and oxidative phosphorylation, and the connection between dysfunctional mitochondria and production of reactive oxygen species (ROS), subsequent studies were conducted to assess levels of oxidative stress following treatment with the small molecule [ 25, 26].
