Sentence examples for subsequent steps as from inspiring English sources

Drug was present during the subsequent steps as well as until fixation.

After the initial step, the C2 domain retained its original diffusion speed in subsequent steps as would be expected, but surprisingly, the full-length protein slowed dramatically over a period of ∼200 ms as shown in Figure 5B.

Incubation with primary antibodies was carried out overnight at 4°C followed by subsequent steps as follows: For pro SP-C/Ki67 co-staining sections were incubated with donkey anti-mouse biotinylated antibody (1∶200) and donkey anti rabbit Cy5 antibody (1∶200) for 90 min at room temperature (RT) sections were subsequently incubated with streptavidin conjugated Alexa 555 (1∶200).

GAPDH was diluted in 5% BSA in TBST (1 10,000 dilution) and incubated at RT for 2 h followed by subsequent steps as described above.

The first six bases of each read were ignored for mutation analysis in subsequent steps as mismatches can be introduced by random hexamer priming during library preparation.

TMA tissue sections (4 μm) were deparaffinized (Tissue-Tek Tissue-Clear Xylene Substitute, Sakura) and re-hydrated according to standard protocols and antigen retrieval was carried out in two subsequent steps, as described previously [ 27].

Any negative branch lengths are corrected in a subsequent step as is the estimation of divergence times for nodes missing such values according to a pure-birth model based on relative clade sizes (see [ 122]).

As illustrated in Fig. 7, Stoeva et al. [125] skillfully used shell-core type functionalized iron oxide NPs (SiO2/Fe3O4 NPs) that electrostatically attract 1 3 nm gold-nanoparticle seeds which act in a subsequent step as nucleation sites for the formation of a continuous gold shell around the shell-core NPs upon HAuCl4 reduction.

β could be bound by the transposase first to initiate the reaction and then used to target the appropriate polymerase in a subsequent step, as in the case of transposases that use a copying mechanism.

For isolation of intracellular biotechnology products, the operation of cell disruption is considered a critical step in product recovery from host microorganism which usually is essential for downstream processing since this step influences the quantity of the desired protein, the ease of subsequent purification steps as well as its biological activity [ 14].

Subsequent steps were as described above for the velocity calibration experiments.