Sentence examples for subsequent sphere from inspiring English sources
Culturing early neoplastic dysplastic keratinocytes (DOKLuc) on nDP modified scaffolds reduced significantly their subsequent sphere formation ability and decreased significantly the cells' proliferation in the supra-basal layers of in vitro 3D oral neoplastic mucosa (3D-OT) when compared to DOKLuc previously cultured on nDP-PHY scaffolds.
On days 7 10, 1st generation spheres were dissociated into single cells, resuspended in the sphere-forming media and observed for subsequent sphere generation.
Serial passage of tumorsphere cultures is known to improve the efficiency of subsequent sphere formation [ 26, 27], and both the shCTRL and shPODXL populations exhibited an approximately threefold increase in the frequency of sphere-initiating cells over three passages.
So deriving d in this way won't justify the use of the subsequent sphere-packing framework.
To determine the number of cells within a sphere that were self-renewing and capable of giving rise to subsequent spheres we used the limiting dilution assay.
After four serial passages, the neurosphere-forming cells retained their ability to form subsequent spheres, however, the spheroid-forming cells after repeated passages became depleted.
In order to make our results more accurate, we performed subsequent generations of sphere forming assays.
Enzymatic dispersion of 2-week-old G1 spheres and subsequent culture revealed that the spheres can be passaged up to seven generations (G7, 3 months) and that the total number of cells increased with each generation.
The cells were cultured on ultra-low attachment plates (Corning Inc., 3471) for subsequent organization into spheres.
To allow for the maximum response and any subsequent leaching, the spheres were given twenty-four houponupon the addition of glucose prior to triplicate fluorescence spectra being taken.
Recently, Yao et al. [31, 32] reported the transformation of fructose to 5-hydroxymethylfurfural through an intra-molecular dehydration process followed by subsequent formation of carbon spheres.
