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When cells reached ∼80% confluency, macrophages were removed from the culture by immunosorting using rat anti-mouse CD16/CD32 Fcγ receptor antibodies (AbD Serotech) and with two subsequent sorts using rat anti-mouse ICAM-2 antibodies (AbD Serotech) endothelial cells were positively selected.
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In their study, a device with a sequence of electrodes in sidewalls along the main channel were used to generate non-uniform electric fields for positioning of particles at different equilibrium points and subsequent sorting into five distinct channels.
Subsequent sorting for ALDH activity (Figure 3F) gave results indistinguishable from experiments from which murine cells were not excluded.
This ubiquitylation is not needed for Golgi-to-endosome transport of Gap1, but is essential to its subsequent sorting into the MVB pathway [9].
Five unbaited pitfall traps were placed inside each median, three of which were randomly chosen for inclusion in the study and subsequent sorting; this design accommodated occasional trap disturbances by natural or human means.
The subsequent sort gates were based on the upper (P3, P4) and the lower (P5, P6) 20% of the SSC-A distributions of the "low" (P1 gate) and the "high" (P2 gate) FSC-W populations (Figure 4A lower left panels).
Under either scenario, subsequent sorting out of adapted hybrid genotypes then occurred postintroduction.
In the specific approach, tBLASTn and subsequent sorting led to 321 contigs.
The signal(s) in Pex3 that mediate its subsequent sorting to the ER subdomain and to peroxisomes remain unclear.
The use of a vital dye staining makes the populations suitable for subsequent sorting and RNA extraction.
These results indicate that the short-chain ubiquitination of AQP2 regulates its endocytosis and subsequent sorting to multivesicular bodies and lysosomes (Fig. 5) (Kamsteeg et al. 2006).
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