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This is an additional factor to consider when deciding to screen a particular compound library for a target, namely, that if the cost of screening in relation to the predicted gain is very large, as indicated by the internal validation of the small training set, then the likelihood of generating an overall gain from subsequent screening of a predicted subset is very low.
The highest xylose uptake was seen in the yeast C. intermedia, and subsequent screening of a C. intermedia cDNA library in an S. cerevisiae HXT null strain identified the GXF1 transporter gene [ 23].
Previously we reported the synthesis of C-terminal peptides via SPOT synthesis and the subsequent screening of a peptide library containing 340 different sequences prepared on a single membrane using S. cerevisiae PFTase (yPFTase) to study its Ca1a2X-box specificity.
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The second series of PKC sensors was identified via the preparation and subsequent screen of a library of fluorescently-labeled PKC peptide substrates.
A subsequent screen of a panel of factors identified several neurotrophic factors that protect photoreceptors in a light-damaged model, including CNTF (ciliary neurotrophic factor), a member of the interleukin-6 (IL-6) family of cytokines [4].
Our overall strategy for optimizing the H3K27-MetBio involved the construction and subsequent screening of 3 iterative libraries; a domain library (lib1), a low resolution linker library (lib2), and a higher resolution linker library (lib3).
No association of this allele with breast cancer was found in a subsequent screening of 133 patients with bilateral breast cancer and 136 controls.
A subsequent screening of local pig farmers showed MRSA prevalence of >20%, which suggested that contact with pigs, at least in that region of the Netherlands, posed a substantial risk of acquiring MRSA (4 ).
Subsequent screening of the whole gene in a cohort of 188 individuals with cervical dystonia revealed five further novel of variants, of which segregation could be demonstrated for two.
A subsequent screening of the entire PRPS1 gene in 16 unrelated probands from X-linked deaf families led to the discovery of two additional missense variants [c.343A>G (p.M115V) and c.925G>T (p.V309F)] segregating with hearing impairment, and associated with mildly-symptomatic peripheral neuropathy.
In the subsequent screening of the three crude extracts, an extract from the Leucopaxillus albissimus mushroom was identified as the only one showing promising activity against either A. baumannii UWL or B. cepacia ATCC 25416 The zone of antibacterial activity of this extract was larger against B. cepacia compared to the zone for A. baumannii.
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