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The second series of PKC sensors was identified via the preparation and subsequent screen of a library of fluorescently-labeled PKC peptide substrates.
A subsequent screen of a panel of factors identified several neurotrophic factors that protect photoreceptors in a light-damaged model, including CNTF (ciliary neurotrophic factor), a member of the interleukin-6 (IL-6) family of cytokines [4].
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This is an additional factor to consider when deciding to screen a particular compound library for a target, namely, that if the cost of screening in relation to the predicted gain is very large, as indicated by the internal validation of the small training set, then the likelihood of generating an overall gain from subsequent screening of a predicted subset is very low.
Previously we reported the synthesis of C-terminal peptides via SPOT synthesis and the subsequent screening of a peptide library containing 340 different sequences prepared on a single membrane using S. cerevisiae PFTase (yPFTase) to study its Ca1a2X-box specificity.
The highest xylose uptake was seen in the yeast C. intermedia, and subsequent screening of a C. intermedia cDNA library in an S. cerevisiae HXT null strain identified the GXF1 transporter gene [ 23].
A subsequent screen of pH values gave an optimum of 7.5.
A subsequent screen of SOS constitutive transposon mutants revealed dnaQ mutants with a substantial but incomplete defect in SOS induction following quinolone treatment (Pohlhaus et al. 2008).
A subsequent screen of the A. nidulans NPK and NPP null mutant collection identified NPKs and NPPs involved in the regulation of CCR and cellulose utilization (Brown et al. 2014).
A subsequent screen of 92 RTS patients revealed that 36 had mutations in CBP and three had mutations in p300 [ 31].
A subsequent screen of 69 diverse cultivars from the world rice collection showed that Cd concentrations in xylem sap were also positively correlated with genotypic variation in grain Cd concentration [ 14].
In a subsequent screen of sera comparing patients with SLE versus controls, it was found that the sera from patients with SLE reacted to acetylated histone H2B and were enriched for other histone PTMs associated with transcriptional repression [ 36].
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