Sentence examples for subsequent round of selection from inspiring English sources

An aliquot of the amplified DNA template (∼1 µg or 20 pmol, roughly one-sixth of total amount of amplicon produced) was used in a transcription reaction to generate an RNA pool for the subsequent round of selection.

There was an increase in phage TUs in subsequent rounds of selection indicating enrichment of selected anti-PLAP phage.

To decrease the background streptavidin binding for the subsequent rounds of selection, the recovered phages were amplified and incubated with streptavidin beads for 15 min, after which the beads were discarded.

Subsequent rounds of selection used DNA collected, amplified, and purified from the previous round as the input DNA.

The selection pressure was increased in two subsequent rounds of selection by decreasing the amount of biotinylated xyloglucan.

It is necessary to obtain ssDNA from double-stranded PCR product prior to the subsequent rounds of selection.

Following RT / PCR amplification in vitro, the most numerous polymerase genes are preferentially cloned and carried into subsequent rounds of selection.

DNA that is eluted in this fashion is PCR-amplified to seed a new, enriched library for use in subsequent rounds of selection.

DNA from these sorted beads was recovered by PCR to generate an enriched library and used in subsequent rounds of selection.

Rescued phage particles were used to test their antigen recognition properties by ELISA or to initiate subsequent rounds of selection using the similar conditions.

PBS (2 ml) was used to recover the phage, and 100 μl of solution was used in the subsequent rounds of selection.