The Radziwiłłs' castle was plundered by the Russian army in 1812 and the subsequent fate of their replica of Szczerbiec is unknown.
This replica was used on subsequent occasions up until 1970 when the original trophy had to be handed back to FIFA.
Specifically, we carried out rotating bending fatigue tests and subsequent observations of the specimen surface by replica technique, fracture surface by scanning electron microscopy, and detailed microstructure in a vicinity of the fractured part by electron backscatter diffraction observations.
Significantly large amount of carbon is introduced into the confined nanochannels of inorganic crystal (zeolite Y) by a pulsed chemical vapor-deposition technique, and the subsequent chemical etching produces a carbon as a negative replica of the inorganic crystal.
Subsequent testing by FIFA, however, confirmed the auctioned trophy was indeed a replica and FIFA soon afterwards arranged for the replica to be lent for display at the English National Football Museum, which was then based in Preston but is now in Manchester.
A strong influence of the microstructure on subsequent fatigue crack growth is observed from interrupted testing with replica records and SEM examination of tested samples, with cracks exhibiting significant tortuosity, at times even appearing to grow approximately parallel to the tensile axis.
The best replica for each condition was used for the subsequent analysis.
Subsequent selection of 150 ZeoR isolates was carried out using replica plating on solid YPD medium containing 200, 500 or 1000 μg ml-1 of Zeocin.
In the most complex cases, the creation of a 3D 1 : 1 replica of the patient's aneurysm and its subsequent implantation in a cadaveric specimen may assist the surgeon in determining how to attack the aneurysm by selecting the best positioning and approach.
In parallel, cells were seeded in limiting dilutions in96-well plates (1, 3, 10 and 30 cells per well; 48 replica for eachcondition) to calculate the CFU-f frequency over subsequent passages.
