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Molecular dynamics based flexible fitting protocols allow subsequent refinement of the atomistic models.
For the subsequent refinement of the preliminary models resulting from both interactive and automatic peak selection, we have implemented a novel simultaneous multi-body docking in Sculptor and Situs that softly enforces shape complementarities between components using the normalization of the cross-correlation coefficient.
The introduction of cardiac troponin (cTn) assays in 1989 was the next major advance, and subsequent refinement of the assays now has the definition of acute myocardial infarction (AMI) centered on it.
In a subsequent refinement of the NS1 analysis, we separated sequences for this protein into two groups, corresponding to the two major NS variants known as Alleles A and B, whose nucleotide sequences exhibit approximately 70% identity [20], [21].
Subsequent refinement of the coordinates and individual B factors were carried out in the Phenix refinement module.
This interval of letting the mean gravity stabilize for 1 h with a subsequent refinement of the slower velocity is repeated until the end of the experiment.
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Although molecular cues and axon guidance are involved in the initial stages of cortical map formation, the resulting maps are rather crude and some form of spontaneous or stimulus-driven activity appears to be necessary for the subsequent refinement of these maps through the pruning of initially exuberant axonal arborisations.
We have established that mutations in the gltA gene encoding citrate synthase (CS) were critical for both potentiating the evolution of aerobic citrate utilization in the Lenski LTEE and for the subsequent refinement of this new metabolic capability.
To predict initial homologous protein sets, mcl (Van Dongen 2008) was used to cluster BLAT results, with subsequent refinement of these sets using hidden Markov models as previously described (Durbin et al. 1998).
Although there have been subsequent refinements of the stratigraphy [15], [16], for convenience we follow Ivany et al. [10] in retaining the Telm designations.
This provided time for data analysis following interviews and subsequent refinements of the interview questions to explore emerging themes and topics.
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