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A series of multiscale simulations of contact formation, indentation, subsequent pulling, and separation between a tungsten indenter and a nickel substrate are performed by means of the quasicontinuum method.
To quantify the extent to which pharyngeal attachment and subsequent pulling forces lead to higher tension near the sensory depression, we compared FRET at the sensory depression region (SDR) with areas outside the sensory depression (non-SDR) in embryos before and after pharyngeal attachment to the epidermis (early comma and 1.5-fold stages, respectively; see 'Materials and methods').
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And yet, he had hung on grimly until a poor tee shot and subsequent pull into a water hazard on the 11th.
Note that in order for this prediction to be accurate, care must be taken to ensure that all subsequent pulls have conditions matching the original one in order to ensure a consistent hot-zone length.
One-mL elution fractions containing the purified GST-GRF fusion proteins were pooled and dialyzed against PBS buffer (20 mM Na-phosphate, pH 7.4, 150 mM NaCl) in order to remove reduced glutathione from the elution buffer which is essential for subsequent pull-down analysis.
Subsequent pull-down assays were performed with TNF1 and TNF1-opt immobilized on agarose beads in order to determine if the increased TNF1-opt E.coli lysate binding is due to general non-specificity or specific binding to a small number of E.coli proteins.
In subsequent pull-down assays, α9A readily precipitated the non-phosphorylated, but not the PKA-phosphorylated protein.
In contrast, a soluble extract of the L3 stage reacted well with the Brugia antibody and was used in subsequent pull-down assays.
It incorporates the polls available for each subsequent day, pulling in additional surveys as it continues toward the current date — at which time all of the polls meeting HuffPost's criteria are being considered.
It incorporates the polls available for each subsequent day, pulling in additional surveys as it continues toward the current date — at which time all of the polls (that meet HuffPost's criteria) are being considered.
Remarkably, in both ndc80Δ 490-510 and ndc80-7A muthets, the end-on attachment was rattachmentblished at 35°C, thus making subsequent end-on pulling infrequent, compared wash wild-type cells.
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