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One year later, fresh leaves of the seedling were collected for RNA extraction, and the subsequent protocols for the transcriptome sequencing followed Chen et al. (2011).
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The current analyses were not planned in the original trial protocol or the subsequent protocol for the adjudication of vascular events.
This paper reports the preliminary development of a test rig and subsequent protocol for its use to acquire the biomechanical properties of the human heel pad.
Ethics approval for the initial protocol was received from the Mater Hospital Human Research Ethics Committee (1709M) on 4th April 2011; subsequent protocols were approved for the 16 month, 2½, and 4-year face-to-face assessments (Ref. #1844M).
We used Krebs buffer (pH 7.4) for subsequent protocols.
Adherence to laminin-coated plates over 2 h was observed, media changed and viable healthy ARVMs used for subsequent protocols.
The vessels were studied at L0 in all subsequent protocols.
Protocols for the TGGE.
Subsequent protocol amendments will be submitted to the Research Ethics Committee for approval, and the other bodies where necessary.
We followed the manufacturer's protocol for the subsequent extraction steps.
As part of a subsequent research protocol for an economic analysis we collected patient utilities utilizing the HUI MarkII/III [ 66- 68].
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