One year later, fresh leaves of the seedling were collected for RNA extraction, and the subsequent protocols for the transcriptome sequencing followed Chen et al. (2011).
This paper reports the preliminary development of a test rig and subsequent protocol for its use to acquire the biomechanical properties of the human heel pad.
After washing with 1 × TBST three times for 10 min, secondary antibodies were added and the subsequent protocol was identical to the western blotting (paragraph above).
Ethics approval for the initial protocol was received from the Mater Hospital Human Research Ethics Committee (1709M) on 4th April 2011; subsequent protocols were approved for the 16 month, 2½, and 4-year face-to-face assessments (Ref. #1844M).
Subsequent protocol amendments will be submitted to the Research Ethics Committee for approval, and the other bodies where necessary.
We followed the manufacturer's protocol for the subsequent extraction steps.
We carried out a specific protocol for the adherence and subsequent biofilm formation on the materials used.
In this context, the paper presents a detailed protocol for the collection and subsequent use of local knowledge in fisheries management planning using geographic information systems (GIS).
In summary, a reliable protocol for the deprotection and subsequent cleavage of DNA and RNA microarrays with EDA was developed using a 3′-succinylated dT phosphoramidite.
Based upon a literature review and available consensus, the working group devised a protocol for the investigation and subsequent clearing, or not, of the C-spine in an unconscious patient.
