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All subsequent procedures were identical with those described for organotypic slice cultures from P8 mice (Gä;hwiler, 1981).
Animals handling and subsequent procedures were in accordance with European [European Communities Council Directive of November 24 , 1986(86/609/EEC)] and Italian laws (D.L. 04.03.2014, n°26) and were approved by SISSA Board for Animal Welfare.
Subsequent procedures were in accord with a previous publication [23].
There was also no statistically significant difference in fluoroscopy time when comparing the first procedure, in which dense nephrograms were present, with subsequent procedures (p = 0.0625) or when normal expected and focally dense nephrograms were detected (p = 0.9055).
Subsequent procedures may involve water treated with probiotics, which help restore the colon with friendly bacteria.
All subsequent procedures were performed on ice, with buffers containing the protease inhibitors (cOmplete EDTA-free) as previously described [21].
After treatment with ALA, subsequent procedures were performed under light-protected conditions.
The buffer throughout purification and subsequent procedures was 0.1 M Tris-HCl, pH 7.6, with 5 mM EDTA.
Subsequent procedures do not improve upon initial efficacy.
Complications, subsequent procedures, and failure rates were compared.
Subsequent procedures were as in the preceding series.
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