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Suitably pure maxillary (MxP), mandibular (MdP), and frontonasal (FNP) samples were placed in separate tubes containing RNAlater (Ambion) and stored at −20°C for subsequent pooling and processing.
For Illumina-based sequencing, GA (Genome Analyzer) and HiSeq instruments were employed to generate data of essentially equal quality, permitting subsequent pooling of the data.
The MLVA assay was easy to perform, consisting of four separate PCR reactions with subsequent pooling of the products, and capillary electrophoresis.
Apolipoprotein (Apo E genotype of selected cases was not considered, since subsequent pooling of samples has the effect of cancelling specific differences in protein abundance due to individual case variation.
The lower reproducibility mostly results from the fractionation of peptides prior to LC-MS/MS analysis, and the subsequent pooling of eluted fractions, which can result in unequal/uncontrolled pooling, as was the case in the present study.
Subsequent pooling of the samples with a ratio of 3 1 1 (wgaDNA:mRNA1 mRNA2) cluster generation and DNA sequencing was performed on a single lane of an IlluminaHiSeq instrument with a 100 base pair paired-end sequencing chemistry.
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Black Friday — and the subsequent pool of days that has spread around it — are significant for consumers because they get a chance to buy items at a deep discount to their normal prices.
This finding has notable implications for predicting the future course of a pandemic because the subsequent pool of those susceptible after initial transmission will diminish (18 ).
Lateral image from contrast oesophagram using Omnipaque-350 (a) demonstrated extraluminal leak and subsequent mediastinal pooling (arrow) of orally administered contrast.
Precisely under these circumstances, the role of fluid mechanics is paramount as multiple zones of the bifurcated regime experience flow stasis and subsequent drug pooling.
Statistical analyses were performed subsequent to pooling the data.
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