Sentence examples for subsequent passage in from inspiring English sources

The virus was isolated by inoculation of patient sera into Aedes albopictus mosquitoes, and subsequent passage in C6/36 cells before determination of genomic sequence.

A subclone of GLM-1 cells resistant to gefitinib (GLM-1R) was isolated after four cycles of a selection treatment consisting of continuous exposure of parental cells to 1  μ M gefitinib for 1 2 weeks, followed by permitting regrowth of surviving cells and subsequent passage in vitro for each.

We therefore measured the ability of IMEC WT and -MYC to grow for subsequent passages in low adherence conditions as mammospheres41.

During subsequent passaging in vitro, progeny of cells with gained chromosome 12, 17 and X are likely to persist and overtake the culture.

A normal (unchanged) cytoskeleton structure could be observed in cells cultured up to 10 subsequent passages in hyperglycemic conditions.

The CD44−CD24+ CSCs isolated from bone expressed a signature of significant enrichment of sets of genes biologically relevant in the process of cancer dissemination that persisted at subsequent passages in the absence of surrounding bone tissue (Subramanian et al, 2005).

The tumour take rate for xenografted GBM biopsies were 96% and remained close to 100% at subsequent passages in vivo, whereas only one of four lower grade tumours engrafted.

Gene expression analysis of individual Brca1 mammary tumors and their subsequent passages in vivo revealed substantial heterogeneity in gene expression [ 40], as predicted by differences in frequency and identity of cancer stem cell populations in cell lines derived from these tumors.

Differences in survival times were maintained after subsequent passages in this mouse model (Table), suggesting that the increased infectivity of sheep BSE cannot be linked to a higher infectious titer in the initial inoculum but must be the outcome of a modification in the pathogenicity of the agent.

Controls cultures Subsequent passage is represented in Figure 1B.

For the in vitro experiment, the cells were use directly for subsequent passage 1 (P1) cultivation either in monolayer or embedded in the hydrogel.