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Subsequent mapping of the site of the cross-link by peptide mapping provides important information concerning the organization of the complex and the trajectory of the nucleic acid components over the surface of the protein.
This is accomplished by first scaling the airway tree model to the volume defined by ventilation imaging followed by subsequent mapping of the terminal alveolar units to each ventilation defect.
This library was used as a reference for subsequent mapping of the tags in the MMSDK analysis.
The parameters determining the largest correlation were selected to be used in the subsequent mapping of the WGSAS.
Subsequent mapping of the sequence data to the optical map allowed for validation of the genome assembly.
PI3K discovery and subsequent mapping of the signaling pathway heralded intense efforts to develop inhibitors targeting the pathway components [ 16– 16].
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The ChIP-seq method is based on sequencing the short 'reads' at the ends of the p53-bound DNA fragments (several hundred base pairs in length) with subsequent mapping of these 'reads' to human genome.
The first three genetic linkage groups of L. angustifolius were assigned to chromosomes using a similar approach, based on hybridization of SSR-rich probes with the BAC library, selection of single-locus clones in BAC-FISH, and subsequent mapping of these clones using BES markers [ 34].
Sequencing runs and subsequent mapping of reads to the respective reference genomes are shown in Additional file 2. In summary, we could uniquely align 147 million out of 161 million reads for annotation purposes (mapping efficiency >91%, Additional file 2).
Using a ChIP-based method, subsequent mapping of chromosomal targets of the nine transcription factors within this expanded core network (that is, three core factors, Nanog-interacting proteins, and Yamanaka's four somatic-cell-reprogramming factors) revealed a positive correlation between transcription factor co-occupancy and target gene activity [ 32].
For three of the strains the plasmid insert positions were established by mapping to the plasmid and subsequent mapping of mate reads to the reference genome.
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