Sentence examples for subsequent library from inspiring English sources

A nested PCR strategy and degenerate primers were employed for the amplification of the gene coding for the alpha subunit of soluble di-iron monooxygenase enzymes from the environmental samples and subsequent library construction.

For subsequent library screening, the mating protocol was followed (Clontech).

Following a clean-up step, tagged DNA was PCR amplified and the subsequent library validated.

Subsequent library preparation followed the standard multi-step Illumina protocol [ 31].

Only samples with RINs ≥ 9 were used for subsequent library preparation.

Total RNA quality (and subsequent library quality) was checked with the Bioanalyzer 2100.

The elegant library he built for the monastery became the model for subsequent libraries throughout 15th-century Italy.

For library 1, which contained the highest concentration of viral RNA, HaRNAV was one of the most highly expressed agents, and was still in the top 100 for pair abundance in subsequent libraries 2 and 3.

Therefore, to obtain more uniform sequencing coverage, we prepared all subsequent libraries from polyA-enriched RNA.

aLibraries 1 to 5 were made using the classic MPSS protocol; all subsequent libraries were made using signature MPSS [75, 76].

An additional Qiagen PCR cleanup was performed and the subsequent libraries were analyzed on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, California, USA) and the electropherograms examined for library and dimer peaks.