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It is known that the exposure of cells to low concentrations of NO can result in protection against challenges from subsequent higher concentrations (Kim et al, 1995).
When 4% ethanol or 1% n-butanol was added, the TMT strain showed the best tolerance among the engineered strains; all subsequent higher concentrations of alcohols yielded no higher tolerance in each of the cytosolic-expressed MT strains.
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Interestingly, yeast pretreated with low doses of H2O2 show an increased resistance to a subsequent higher concentration of hydrogen peroxide [47] due to the activation of detoxification genes that are controlled by the transcription factor Yap1 [48].
Pretreatment was followed by a subsequent higher concentration of RB (Challenge) as indicated under light.
In the cumulative protocol, which mimics the variable holding potentials in voltage-clamp experiments in single VSMCs, the segments were depolarized to the subsequent higher K+ concentration without return to 5.9 mM K+.
In subsequent experiments utilizing higher concentrations of inoculum and shorter cooker time periods all plates were effectively sterilized by 30 minutes in the cooker (figure 3c and d).
For example, in breast cancer cells lines, low concentrations of docetaxel induced aberrant mitosis and subsequent necrosis, whereas higher concentrations induced mitotic arrest and apoptosis.
In the 6/33 cells that did not respond to 30 μ m serotonin, subsequent application of higher concentrations (60 120 μ m) failed to elicit significant effects.
Nevertheless, subsequent studies should test also higher concentrations of methylglyoxal.
Subsequent experiments focused on how higher concentrations of BTE affected HSV-1 infectivity.
Recombinant GST-MDA-7 protein has also been shown to possess anti-proliferative properties as observed in previous work in RCCs., Even in nanomolar concentrations, the protein facilitates growth arrest attributed to the above mechanisms, and these effects are enhanced further to the point of almost complete growth inhibition and subsequent apoptosis at ~20-fold higher concentrations.
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