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Trib2 was first identified as a Neurogenic Locus Notch Homolog Protein homologue 1 (NOtargettarget in murine T-ALL [ 8, 25] and subsequent gene expression studies show that TRIB2 is highly expressed in T-ALL [ 21].
Our technique guarantees the isolation of sufficient high quality RNA obtained from specific cell populations of the brain expressing soluble fluorescent marker, which is a critical prerequisite for subsequent gene expression studies by microarray analysis or qRT-PCR.
We demonstrate high quality of the isolated RNA from a defined cell population, the periglomerular cells of the olfactory bulb, and show the usefulness and specificity of the technique for subsequent gene expression studies by quantitative real-time PCR and microarrays studies.
From stage 3 and onwards, the embryos were picked individually and sorted according to developmental stage to increase the signal to noise ratio in subsequent gene expression studies.
Subsequent gene expression studies have identified prognostic transcriptomic profiles that appear to be pertinent for the prognostication of short term relapses, specifically in estrogen receptor positive breast cancer [ 12, 13].
Radiation doses for subsequent gene expression studies were chosen based on the cell killing data so as to cover the range of RBEs between Fe/γ-ray and Si/γ-ray, such that we can compare gene expression differences at the same doses as well as at or near the same biological effect, that is, cell killing.
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This molecular heterogeneity has been further confirmed and improved in several subsequent gene expression-based studies [ 27].
Human brain RNA (Clontech, Saint-Germain-en-Laye, France) was converted to cDNA and used to generate standard curves for subsequent voltage-gated Ca2+ channel gene expression studies.
Moreover, all gene expression studies and subsequent analyses were performed with strain COL.
Ideally, gene expression studies will allow scientists to identify changes in transcription associated with exposure and subsequent risk of developing disease.
A cross-sectional descriptive study of hormone levels is also important, to avoid misclassification of subjects in a subsequent gene expression analysis of the same material.
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