Sentence examples for subsequent gene expression profiling from inspiring English sources

Individual RNAs were analyzed for RNA quality by Bioanalyzer Chips (Agilent Technologies, Santa Clara, CA) and only RNAs with a RIN of 9.0 or higher we used for subsequent gene expression profiling.

Stimulated cartilage-like cultures were used for subsequent gene expression profiling with oligonucleotide microarrays.

The Meis1-GFP transgene specifically marks mesangial cells within the glomerulus, allowing their purification through fluorescence activated cell sorting (FACS), and subsequent gene expression profiling with microarrays.

An additional sample of rice grain size (approximately 30 mg) was submerged in 2 ml RNAlater® (Ambion, Austin, TX, USA) for RNA extraction and subsequent gene expression profiling.

Since the pyronin reagent reacts with RNA, we worried that MGP might damage the RNA and compromise the subsequent gene expression profiling.

Purified total RNA samples were stored frozen at -80°C until needed for quality control (QC) analysis and subsequent gene expression profiling.

Accordingly, we treated different doses as replicates in our subsequent analysis of gene expression profiling.

At the end of GC treatment, supernatant was discarded and the cell monolayer was kept frozen at -80°C for subsequent total RNA extraction and gene expression profiling.

Our gene expression profiling data with subsequent analysis of the affected biological pathways as well as utilization of the locus-specific gene expression tool suggested a potential impairment of protein phosphorylation genes related to cytoskeleton-mediated growth cone anatomy in Cln1 and Cln5 deficient mice.

However, due to the low number of endothelial cells collected (mean of ∼97 cells in the current study) and the subsequent low RNA yield these types of material have never been practically accessible for global gene expression profiling.

high-coverage gene expression profiling.