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Subsequent gene expression analysis identified 998 differentially expressed transcripts and functional analysis revealed that metal- and ion-homeostasis pathways are likely to be the most important mechanisms contributing to the metal tolerance exhibited by this population.
The 2 genes deemed most suitable for the experimental design were RPL19 and YWHAZ, which were selected for subsequent gene expression analysis.
As a result, RNA of shorter size would be isolated preferentially over longer RNA, which would compromise the subsequent gene expression analysis.
However, there is a clear need for a reliable technique to separate specific in vivo fluorescent neural cell populations for subsequent gene expression analysis.
The principal challenge with this technique was to obtain a significant quantity of RNA from SOPs and to ensure that the integrity of the RNA after laser microdissection was sufficient for subsequent gene expression analysis such as quantitative real time PCR and microarrays.
Subsequent gene expression analysis showed that genes in these regions exhibit different expression patterns.
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Subsequent global gene expression analysis revealed that 5-Aza-2-deoxycytidine treatment leads to (abnormal) expression of several cancer related genes.
Our network analysis would suggest Centg3 and Elavl4 are important candidates for perturbation and subsequent global gene expression analysis for further network resolution, as they are both highly connected nodes and little is known about their function in the developing telencephalon.
Subsequent genome-wide gene expression analysis (see materials and methods) revealed that both compounds induced significant transcriptional responses).
Our results strongly suggest that identification of probes with detectable cross-species hybridization signal and subsequent restriction of gene expression analysis to these probes can be applied to multiple related species for which gene arrays are not commercially available.
Our technique guarantees the isolation of sufficient high quality RNA obtained from specific cell populations of the brain expressing soluble fluorescent marker, which is a critical prerequisite for subsequent gene expression studies by microarray analysis or qRT-PCR.
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