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Thus 30 µM of LA was used in all subsequent experiments of this study.
Based on comparable HI titers obtained by the two erythrocyte species as well as our convenience to obtain goose erythrocytes; therefore, goose erythrocytes were employed in the subsequent experiments of our HI assay.
To better quantify the extent of reduction and to compare the degree of CMV inhibition by different artemisinin derivatives all subsequent experiments of CMV inhibition were performed using the highly sensitive luciferase assay and the pp28- luciferase expressing CMV.
Based on these results, we decided to shorten the incubation time to 6 h in the subsequent experiments of microbial-assisted oil extraction.
We used the IC50 of G-1 for PC-3 cells, 1.02 × 10−6 M, in the subsequent experiments of this study; the IC50 for DU145 cells was 3.21 × 10−6 M. The difference in these IC50 values was statistically significant (p<0.0001), suggesting that PC-3 cells were more sensitive than DU145 cells to G-1.
Subsequent experiments of DNA transfection and STING dependent signalling does not prove the above point, since the authors do not address their original question (subsection "ISG15 is induced via the Cytosolic Surveillance Pathway (CSP) following sensing of cytosolic DNA") to find the PAMP that is necessary in inducing ISG15.
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Therefore, in subsequent experiments on the operation of the car audio system, the number of HSOM layers is determined by the total number of songs.
Littermate Opn4Cre/+ R26iDTR/+ and WT mice were used in all subsequent experiments for acute ablation of mRGCs.
These cell lines were used in many of the subsequent experiments in view of our previous experience of their reliable in vitro behaviour.
Therefore, the littermates of the cDKO mice were used as a control in subsequent experiments, irrespective of their genotypes.
For subsequent experiments, three of these were selected, representing different methodologies (AnaP, EznF, EriP).
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