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"If it is necessary to record both first-level and subsequent level enumeration in the holdings statement, repeat all levels of enumeration at the beginning and end of each range held". (ANSI p. 29) In the following example, a gap occurs in the subsequent enumeration level of v. 2, so both ends of the range in the first 866 repeat all levels of enumeration, even though v.1 is complete.
One possibility is that when the number of to-be-enumerated items exceeds the subitizing range, one lateralized quantity is enumerated first while the other is held in short-term memory before being processed for subsequent enumeration and integration with the first value.
Effectively, the DEE-based pruning stage reduces the base q of the enumeration exponent, typically making the subsequent enumeration of the remaining unpruned conformations computationally feasible.
This pruning step aims at significantly reducing the number of unpruned rotamers that must be considered in the subsequent enumeration stage.
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Age-specific population estimates for the era before the KHDSS began were extrapolated using a log linear model of age-specific data based on subsequent enumerations.
For quantification of L. monocytogenes in clinical tissues after Matrix-Lysis, the remaining pellet was either plated onto selective agar plates for viable cell quantification or DNA extraction was performed for subsequent qPCR enumeration.
There are no gaps in volumes 3, 4, and 5, so the subsequent levels of enumeration are eliminated when the compressed form is recorded as a summary statement.
At the time of sacrifice the lungs were flash frozen in liquid nitrogen, ground into a fine powder, extracted by Triazol®, quantified and stored at −70°C for subsequent microscopic (cyst enumeration) and quantitation of the P. murina large subunit ribosomal RNA gene (mtLSU) message levels [44].
Cells were detached after 72 h by standard trypsin/EDTA treatment, collected by centrifugation at 500×g 4°CC, 5 min), washed twice in PBS supplemented with 5% FCS (4°C), and resuspended in PBS plus 5% FCS (4°C) for subsequent fluorescent cell enumeration using the FL1 channel (530/30 nm filter) of a FACSCalibur cytometer (Becton Dickinson, Franklin Lakes, NJ, USA).
The developed 3D-QSAR models were prospectively used to predict the affinities of new compounds designed through R-group deconvolution starting from the core chemical scaffold and subsequent virtual combinatorial library enumeration.
If adults were not present at the time of enumeration two subsequent appointments were made before the adult was recorded as absent.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com