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The oligonucleotides were designed to constitute a double-stranded DNA fragment containing the P AOX 1 promoter core region with EcoRI- and NheI-compatible ends when annealed for subsequent easy cloning into the pPPE20 vector.

Plasmid pPPE50 was used when constructing the plasmid library LU, and the construction of this library is identical to that of the LC plasmid library, except that the oligonucleotides were designed to constitute a double-stranded DNA fragment containing the P AOX 1 promoter core upstream region with KpnI- and EcoRI-compatible ends when annealed for subsequent easy cloning into the pPPE50 vector.

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The subsequent easy-does-it birdie putt took him to 6 under.

As the child gets older, the bone will become harder and more stable with subsequent easier fixation of the distractor.

In contrast, a longer TTD after AC chemotherapy in patients with non-TNBCs, mainly of the HR+ subtype, may be due to the relatively slower tumor growth characteristics and additional benefit from hormonal therapies with subsequent easier disease control than for TNBCs.

Once inside a network, Code Red II then set about creating a secret backdoor to allow subsequent attacks easy re-entry to execute malicious commands from afar.All told, half a dozen main forms of attack are used on networks today.

There were widespread expectations that – with the great financial crisis having had its origins in the US, let alone the subsequent unorthodox "easy money" policy - the "exorbitant privilege" of the dollar in the financial networks linking the Brics into global production and trade would be undermined.

The more than $100 million Williams will end up earning in his career makes any subsequent choice easier.

Every time a film is shot in another state, moreover, the locals pick up skills that make subsequent shoots easier.

These junctions put the player in a very interesting position in terms of narrative, control and identity: do they choose the route they think is morally better, or that they think Serene himself would go for, or do they select what they believe could make the subsequent missions easier to complete?

According to Fox et al. [22], ideally in orthodontics, an adequate bond should fail at enamel composite interface (ARI = 0), as this would make as this would make deboning and subsequent polishing easier.

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