To separate the backplane reflection from all other sources of loss, T i is factored out from all subsequent data; the resulting values are equivalent to the normalized output power with respect to the amount of power entering the device P′ in.
Assigning a value equal to the total number of cycles run causes difficulties in subsequent data analysis as the value will vary dependent on cycling parameters, and is often identified as an outlier value by normal statistical analysis.
The fluorescent intensity value used in all subsequent data analysis was the maximum value of the fluorophore emission for each spectrum collected.
Data analysis was optimized by imputing missing intensity values and subsequent data normalisation.
During model selection, five patients from the Glasgow dataset and one patient from the London dataset were excluded from subsequent data analysis due to missing values, leaving 79 and 114 patients in each group, respectively.
Following primer optimisation a baseline Ct was identified for each primer set, which was used when analysing subsequent data (R, slope and threshold cycle values are shown in Table 1).
However, for data points classified as outliers, ISO guidelines recommend rejection of the value before the subsequent data analysis, unless sufficient justification is given to retain it.
The frequency distribution of echo intensities (Gray units) for the whole image was then calculated and the mean value retained for subsequent data analysis.
Only v values were used for subsequent data interpretation.
The data was then extracted and the single channel normalized values were used in subsequent data analysis.
