In this way, users can arrive at a common residue numbering scheme to help ensure accuracy in subsequent construct design.
Measuring satisfaction by means of a VAS is relatively crude and possibly influences the subsequent construct validity for which it was used.
Any question that was a source of investigator disagreement was added to the Index, with the understanding that subsequent construct validation research would clarify the performance of various items.
This suggests that miR-155 flanking sequences are important for miRNA transcription, stability and/or Drosha processing and we therefore used the complete miR-155 cassette in all subsequent constructs.
This suggests that internal piggyBac sequences missing from the minimal vectors are essential for efficient re-mobilisation of genomic piggyBac insertions, and we therefore used a full-length piggyBac vector (p3E1.2) for our subsequent constructs (Fraser et al., 1995).
This plasmid was then modified to introduce the 21 amino acid C-terminal signal sequence from Y21, a fungal acid protease: the putative ω-site was mutated to alanine in this and all subsequent constructs to optimize continuity following anchor attachment.
Subsequent mannequins constructed of plastic and papier mache were more durable, lightweight, and flexible, making them easier to imbue with lifelike gestures.
Subsequent trials were constructed using the same process with the remaining 20 sounds, until the set was empty.
In it and in subsequent writings, Yaginuma constructed a future history of the development of Japan's spaceflight program.
Where Athens, Rome and subsequent European empires constructed their institutions to prevent class antagonism from devolving into class war, the United States built a legal system that required broad economic equality to function.
In a subsequent step, we constructed another network, connecting human host proteins if they significantly shared directly interacting viral proteins (P<0.01).
