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2500 genes from MCF10HER2 cells, with 1.7 or greater expression fold-change at any time point in the series, were included in subsequent analysis to identify transcripts that are differentially expressed in MCF10HER2 cells compared to MCF10A.
Samples were collected after each step in the n-butanol challenge for subsequent analysis to identify the genes that are enriched or depleted in the presence of n-butanol.
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Solexa technology was used to perform high throughput sequencing of the library and subsequent bioinformatics analysis to identify novel miRNAs.
Firstly, we made use of the CLuster ANalysis of Sequences (CLANS) software, which uses the Basic Local Alignment Search Tool (BLAST) and a subsequent similarity analysis to identify similar subtypes [ 84].
Here, we have utilized microarray and subsequent functional analysis to identify a new transcription factor, BBX, which regulates odontoblast differentiation of human DPSCs/progenitors.
Our strategy utilises this phenotypic analysis for subsequent QTL analysis to identify genetic loci associated with FD, utilising two related populations in which individuals segregated for susceptibility to FD.
A small RNA library from Amur grape was constructed and Solexa technology used to perform deep sequencing of the library followed by subsequent bioinformatics analysis to identify new miRNAs.
Nevertheless, the whole data set or the descriptive subset of genes selected can be used for subsequent mechanistic analysis to identify compound-specific mechanisms or subsets of genes specific for a particular mode of action.
Subsequent cytoscape analysis to identify common cellular functions highlighted two major pathways predictive of cytotoxic response to JQ1; one associated with ATM and related double-strand break repair genes, and the other linked with the cell cycle inhibitor CDKN1A (Supplementary Figure 2).
The majority of the sequencing however, was carried out subsequent to microarray analysis to identify genes that demonstrated differential expression profiles across the moult cycle.
Our subsequent in silico analysis to identify potential interacting partners of the LIN-2/7/10 LIN-2/7/10ghlighted the DAF-2 RTK as a potential LIN-2 binding partner.
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