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Reaction flux analyses were carried out and it was found that fuel H-atom abstraction reactions forming the 4-phenylbut-4-yl radical, and its subsequent addition to molecular oxygen, is the primary source of reactivity in the low-temperature regime.
The cells were then counted using a hemocytometer and the appropriate number suspended in 50 μl DMEM for subsequent addition to the multiwell HSF cultures.
We found that Knochel's iPrMgCl·LiCl complex (the "Turbo Grignard") allowed for mild Br/Mg exchange of tert-butyl ester 13 or 14 and subsequent addition to 12 in reasonable yield (56%and47%7%, respectively, Scheme 1).
Iron oxide-based magnetic particles are prepared by slowly adding an inorganic precursor of iron (III) chloride, dissolved in aqueous medium with the subsequent addition to the oily medium, followed by the addition of pH regulators [ 13– 17].
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If we type the basic information (which we have to to relay it as soon as possible), the auto-refresh then sometimes fails to pick up any subsequent additions to that block of information.
Subsequent additions to Caernarfon were not major, and what remains of the castle is substantially from the Edwardian period.
This was to be followed by all subsequent additions to the Gallery for a century, resulting in a building of clear symmetry.
Subsequent additions to the Board of Trade Building moved the agricultural and financial trading floors out of the original trading room and into new spaces in the additions to the building's rear in the 1980s.
This original design has withstood the test of time: the repetitive formula of the façade was continued during the subsequent additions to the palazzo, and its influence can be seen in numerous 16th-century imitations and 19th-century revivals.
Subsequent TCEP addition to PABA/NO treated HL60 cells did not reverse the drug-mediated sulfhydryl modifications (data not shown) indicating an absence of disulfide bonds and perhaps, corresponding with cell surface protein thiol nitrosylation.
As shown in intermediate 3b, coordination of ZnPh2 with the Lewis basic central oxygen atoms of the BINOL unit should have activated the subsequent phenyl addition to the aldehyde.
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