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Because close monitoring for up to 72 h is recommended for patients who experience an IR [ 10], the time interval for identifying an IR included the day of cetuximab administration and the subsequent 2 days.
In brief, 2 days after neuron plating, 1 µM cytosine arabinose (Sigma) was added to the neuronal culture medium to inhibit glial growth for the subsequent 2 days, then replaced with the normal culture medium.
By 3 dpf, the ISV pattern is largely established, with limited remodelling over the subsequent 2 days.
However, whereas controls (Fig. 3 a ) and DA-depleted animals (Fig. 3 b ) lost this bias over the subsequent 2 days, 5-HT depleted animals did not.
In the PDGF plus Tat group of mice, PDGF was first microinjected for two days followed by HIV-1 Tat microinjection for subsequent 2 days.
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Exendin-4 injection was started immediately after crush injury and was repeated every day for subsequent 14 days.
Cells were treated with 30 and 300 n AZD1152-HQPA for 1 day with or without a subsequent 1 3 days wash out.
Cells were fixed in 10% formaldehyde on the day of plating and daily for the subsequent 4 days.
Since GEM administration scheduled on day 15 was not possible within the subsequent 4 days in six patients, the dose at this level was regarded as the MTD, and the level two dose was considered as the recommended dose.
The intermediate monocyte population remained elevated for the subsequent 7 days after AMI and trended to background control levels by day 30.
The injured intestinal villi were gradually repaired during the subsequent 18 days of the experimental period.
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