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The small number of microdissected cells yielded a suboptimal amount of RNA that could be used as input for the Nugen SPIA amplification.
Since a consistent amount of fat is injected each time, there are no "empty passes" of the cannula in which a suboptimal amount of fat is injected.
As shown in Figure 6D, transfection of a suboptimal amount of miR-124 mimic decreased LPS-induced TNF-α production by ∼25%, which was not further decreased by knockdown of TACE.
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Together, we reveal a mechanistic link between NOTCH2 activation and robust BCR responses to otherwise suboptimal amounts of surrogate antigen.
We immunized wildtype and anti-ASGM1 antibody-treated mice with suboptimal amounts of plasmid DNA to more closely simulate the real-time situation during human vaccination and to increase Treg effects compared to controls.
The increased binding of C3 to the surface of S. pyogenes ΔdltA mutants, their increased interaction with PMN and their reduced ability to grow in whole human blood are all phenotypes associated with S. pyogenes that express suboptimal amounts of M protein.
In Xenopus, co-injection of suboptimal amounts of Sdc4 and Vangl2 morpholinos resulted in a significantly greater proportion of embryos with defective neural tube closure than each individual morpholino alone.
The frequency of defective embryos produced by injection of suboptimal amounts of Sdc4 morpholino (1-4 ng) was significantly greater when xVangl2 was overexpressed by co-injection of synthetic mRNA (Fig. 5G).
Co-injection of suboptimal amounts of Sdc4 and Vangl2 morpholinos (xSdc4-Mo, xVangl2-Mo) resulted in a significantly greater proportion of embryos with defective neural tube closure than the individual morpholinos alone (P<0.001; Fig. 2D).
TCF/β-catenin transcriptional activity was also tested using the TOP/FOP reporter system in cells co-transfected with suboptimal amounts of either the p110 CUX1 or GLIS1 effector plasmid, or the combination of both.
We previously reported that the DNA damage response was the primary transcriptional response that separated thymine starvation leading to thymineless death (TLD) in thyA auxotrophs from the thymine limitation (TLM) wherein cells were supplied with suboptimal amounts of thymine [ 37].
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