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The raw data of 16S rRNA gene Illumina sequencing has been submitted to sequence read archive (SRA) of NCBI database and the accession number was SUB2165181.
Approximately 192 clones from each clone library were submitted to sequence analysis.
Bands were visualised under UV light, eluted and submitted to sequence analysis.
All bands were excised from the gels, submitted to sequence analysis and a consensus sequence compiled.
This approach yielded between 40 and 50 millions of reads that were further submitted to sequence mapping on mouse reference genome mm10 with CASAVA 1.8.2 software.
The protocol that generated the highest yield of DNA was used to create a complete, new library of 2 kb inserts, and the library was submitted to sequence analysis using the protocols previously cited.
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Twenty-four of these spots were submitted to sequencing by mass spectrometry and three different categories of proteins were identified: lipocalins, cuticle proteins and serpins.
DNA concentration and purity were determined in a spectrophotometer BioPhotometer plus (Eppendorf, Hamburg, Germany) and submitted to sequencing reactions.
249Ser mutation was found in 21/74 samples (28%) by RFLP and all samples were also submitted to sequencing.
Colony PCR amplicons were treated with ExoSAP and submitted to sequencing by using the listed plasmid primers.
The Carba NP test can efficiently indicate the strains to be further tested by PCR or submitted to sequencing for a detailed identification of the carbapenemase genes.
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