Exact(15)
The nanotube surfaces submitted to reverse polarization, presented an improved cell adhesion and proliferation compared to smooth titanium.
PolyA+ submitted to reverse transcription without the actual RT enzyme were used as negative control template.
50 µl reactions were submitted to reverse transcriptase for 30 minutes at 50°C, followed by PCR activation step at 95°C for 15 minutes.
To determine the host gene-response to infection, total cellular RNA was extracted at 24 hpi and submitted to reverse transcription in the presence of 33P.
The batches were dried, dissolved in 10 µl of 0.1% TFA/3% acetonitrile and each submitted to reverse phase chromatography as described.
The final products were again gel purified by size fractionation and submitted to reverse transcription reaction using the RT primer (5'-TTTT CTGCAGAAGGATGCGGTTAAA-3'; bold, PstI site).
Similar(45)
The digest was submitted to reverse-phase clean up in 500 mg C18 cartridge.
The resulting solution was clarified by centrifugation, and the supernatant was further submitted to a reversed-phase chromatography on a C8 Discovery BIO Wide Pore (25 cm × 4.6 mm × 10 μm).
An application has been carried out on a hard steel metal submitted to a fully reversed torsion and bending loading.
Sequences that were submitted to INSD as reverse complementary were identified and corrected using Nilsson et al. [46].
Total RNA from the same isolation that was submitted to Agencourt was reverse transcribed using the SMART RACE cDNA Amplification Kit (Clontech Laboratories, Inc., Mountain View, CA) per manufacturers' recommendations.
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