Exact(4)
For each cultivar, we started with 1 μg (ds) cDNA that was submitted to fragmentation using a nebulization method (Roche).
Each selected precursor was accumulated for 50 ms and submitted to fragmentation with N2 as the collision gas.
Eventually, a mass chromatogram of the corresponding authentic standard and the sample were acquired and the peak submitted to fragmentation in a MS/MS experiment.
Non-indexed paired-ends (PE) libraries were carried out with an initial input DNA of 3 μg by following the Illumina Paired-End DNA Sample Prep protocol (Part # 1005063 Rev.D, February 2010) with some modifications: 3 μg of Genomic DNA were submitted to fragmentation by using Adaptive Focused Acoustics (AFA) process from Covaris technology (S2 Focused-Ultrasonicator).
Similar(56)
The product ions were then submitted to further fragmentation in the same conditions, until no more fragments were observed.
For these purposes, two aqueous solutions of chitosan (DD = 90 and 95%) were submitted to controlled fragmentation by ultrasonication.
This acquisition mode is a parallel process where all MS signals are further submitted to MS/MS fragmentation without any precursor selection.
This plant exclusively grows in saline low grounds, which are relict environments within the study area and submitted to a degree of fragmentation comparatively much higher than that experienced by the habitats occupied by the esparto grasshopper (Ortego et al. 2010).
Under the assumption of neutrality, we simulated an equilibrium configuration, that we subsequently submitted to several scenarios of environmental fragmentation.
The resulting peptide mass and the associated fragmentation spectra were submitted to GPS Explorer workstation equipped with MASCOT search engine (Matrix Science Inc). to search the National Center for Biotechnology.
Identification of the resulting peptide mass and the associated fragmentation spectra were submitted to GPS Explorer workstation equipped with MASCOT search engine (Matrix Science, London, UK) to search the non-redundant database of National Center for Biotechnology Information (NCBInr).
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