These sequences were used in the database of National Centre for Biotechnological Information (NCBI) (BLAST, http://blast.ncbi.nlm.nih.gov/) via BLASTN option [20] for possible matches, and thereafter submitted to GenBank for accession number.
The 24 rats were thereafter submitted to a 12 hrs.
Proteins were thereafter submitted to an overnight electrotransfer on a polyvinydilene difluoride Immobilon®-P transfer membrane (Millipore) at 4 °C.
Proteins were thereafter submitted to an electrotransfer on a polyvinylidene difluoride Immobilon®-P transfer membrane (Millipore) during 1 h and 30 mins at 100 V at 4 °C.
As recommended, it was thereafter submitted to the Commission nationale de l'informatique et des libertés (CNIL; http://www.cnil.fr/english/the-cnil/operation/ c1556) which ensures the protection of personal data.fr/english/the-cnil/operation/ c1556
In group AB, the calvaria defect was filled with autogenous bone obtained from ground calvaria bone harvested with the trephine; the same was performed for group ABL, which was thereafter submitted to LLL application.
These hospital priorities/budget allocations were thereafter submitted to a hospital management board, whose membership included external stakeholders such as the community, for approval (Kapiriri and Martin 2006; Kapiriri et al. 2007; Gordon et al. 2009).
Single-walled carbon nanotubes (SWCNT) synthesized using the HiPco® process and purified thereafter were submitted to two covalent functionalization processes: i) a mild oxidation in a concentrated HNO3 solution using microwave irradiation and ii) a radical functionalization to graft methoxyphenyl groups.
In summary, the results of this survey provide unique insights into the decline in the number of foodborne outbreak reports submitted in 2009 and thereafter.
