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Throat swabs were submitted for virus isolation.
Both isolates were submitted for virus isolation to the Animal Disease Diagnostic Laboratory of Purdue University.
Of the 927 serum samples, 282 were submitted for virus isolation and RT-PCR.
Fresh tissue specimens were submitted for virus isolation, reverse transcription-polymerase chain reaction (RT-PCR), and bacterial culture.
During the epidemic, 311 samples from 175 clinically diagnosed HFMD case-patients were submitted for virus culture.
In Sweden, 18%% of feline clinical samples submitted for virus isolation to the National Veterinary Institute during the year 2000 tested positive for FCV, while healthy carriers of FCV in Swedish catteries were estimated at 2.6%% [ 10].
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All cloacal and tracheal swabs from 10 dead and 10 live birds submitted for influenza virus exclusion were positive by an influenza A matrix gene quantitative real-time reverse transcription PCR (8 ), and virus was detected at various levels (cycle threshold [Ct] 15 37).
At the discretion of the attending physician, some children had respiratory tract samples submitted for respiratory virus culture (RSV, influenza, parainfluenza, adenovirus).
The clinical signs, findings at necropsy and absence of other pathogens corresponded well with EMPF, and thus samples from lung tissue and tracheal wash were submitted for further virus examination.
A concern was raised about whether the vaccine (which contained a live, attenuated strain of CPV) could have caused the disease and so further faecal samples from the affected pups were submitted for laboratory virus isolation and identification.
In order to better understand the burden of adult disease due to RSV, we undertook a retrospective study of hospitalization due to RSV infection in adults in four acute care hospitals in Toronto, Canada, during a winter season in which routine testing for RSV was added to all specimens submitted for respiratory virus testing.
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CEO of Professional Science Editing for Scientists @ prosciediting.com