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Initially 8 clones were submitted for sequence analysis per positive sample.
This repeat alignment block was then duplicated (side-by-side) before being submitted for sequence logo generation.
Each domain match was submitted for sequence similarity searching against the Swiss-Prot/TrEMBL database [ 116] to determine whether they matched other sequences with the same domain.
Counties labelled with # had samples submitted for sequence typing with CTs above the cutoff for identification as positive in this study.
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Enriched, cleaned product was diluted to 5 pM and submitted for sequencing on an Illumina GAII sequencer at the Center for Genome Research and Biocomputing (CGRB) at Oregon State University (Corvallis, OR, USA).
Enriched, cleaned product was diluted to 5 pM and submitted for sequencing on an Illumina GAII sequencer at the University of British Columbia (Vancouver).
The PCR products submitted for sequencing.
The amplicons were purified with a Universal DNA Purification Kit (TIANGEN, Beijing, China) and submitted for sequencing (Invitrogen, Shanghai, China).
The products were also submitted for sequencing for confirmation of the mutation (data not shown).
When the level of enrichment reached a plateau, pools of interest were submitted for sequencing.
PCR products were submitted for sequencing at Cogenics (UK) using the ABI 3730 xl platform with the TN3 forward and TN4 reverse primers.
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