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For each submitted alignment job, the server will assign a job ID.
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In the case of submitting alignments, they are accepted in FASTA and SELEX formats.
Scripts written in the Python programming language were used to submit alignments to GAP and parse the resulting output.
These clone sequences were then submitted for alignment by the NAST aligner at greengenes (greengenes.lbl.gov) [31].
In order to identify the specific amino acids that allow the discrimination between the two mitochondrial RF1 homologous subfamilies, we submitted our alignment to Sequence Harmony [ 25] and selected the positions that are, in the vertebrates, perfectly conserved within mtRF1 and within mtRF1a, but that are different between them.
A phylogenetic tree was constructed by submitting the alignment in Fig. 3 to the "Mr. Bayes" software ([32], using a "mixed protein" model).
Diverse conformations within 20 kcal/mol energy range were generated and submitted to the alignment procedure.
Conformations within 20 kcal/mol were submitted to the alignment procedure.
Where, a training set representing the most active lead analogs [12, 13] was selected, energy minimized and submitted to flexible alignment for analyzing the shared spatial arrangement of their pharmacophoric features.
To further appreciate subgroup-specific traits, both major phylogenetic subgroups of LmSIDERs were separated and submitted to independent alignment optimization.
The selected clades from functional divergence were submitted as nucleotide alignment in fasta format to the Selecton server for analysis of the non-synonymous (dN) versus synonymous substitution (dS) ratio [ 19].
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