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Failure to start ATT was largely due to early loss to follow-up or death which occurred between submissions of samples and result delivery.
A search of AHL's database was performed to identify other S. pseudintermedius and S. delphini isolates for all submissions of samples from equids during January 2011 August 2012.
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Our data indicate the importance of linking patients to care after submission of samples for testing.
Seroprevalence in each subsequent round of testing was artificially skewed toward higher values as a result of selective and repeated submission of samples drawn from previously seropositive hounds.
The success of treatment was monitored using preputial washings into TEM and submission of samples to the diagnostic laboratoryf for Campylobacter spp culture and FAT.
Culling infected birds in each flock was generally completed 1 2 days after the virus was confirmed by virus isolation (confirmatory diagnosis took ≈2 8 days after submission of samples).
No virus isolation was attempted on brain specimens because all were received in formalin, despite guidelines for submission of samples in glycerol saline.
Cell suspensions were sonicated with three pulses at 20% amplitude, and vortexed once more shortly before submission of samples to FACS analysis.
Passive surveillance is driven by laboratory submission of samples after outbreaks of respiratory disease, whereas active surveillance is based on purposely collecting and screening field samples regardless of clinical status.
Secondly it is likely that the Farmfile disease data introduced an unknown degree of selection bias given that the VLA scanning surveillance system relies on the voluntary submission of samples or specimens usually by private veterinary practitioners.
We hope there will not be any logistic problems with the submission of samples from the health care centers to the laboratory for culture since we will be personally responsible for this task.
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